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目的:研究人参皂甙Rh2对H_2O_2损伤保护作用及与GABAA受体的关系。方法:采用小鼠制备离体海马脑片,随机分为正常组、模型组、GABA组、人参皂甙Rh2(20μmol/L、40μmol/L、60μmol/L)组、荷包牡丹碱组、荷包牡丹碱+人参皂甙Rh2组,每组20张脑片。采用碘化丙啶(PI)染色法测定H_2O_2损伤后海马CA1区细胞活力变化,免疫组化方法观察细胞色素C表达,电生理技术记录顺向群峰电位(OPS)观察突触传递变化。结果:人参皂甙Rh2(40μmol/L、60μmol/L)可明显提高H_2O_2损伤后海马CA1区细胞活力,降低海马CA1区细胞色素C表达,海马脑片OPS恢复率和OPS恢复程度亦明显提高。人参皂甙Rh2的保护作用和外源性GABA作用相似并可被GABAA受体抑制剂荷包牡丹碱所阻断。结论:人参皂甙Rh2可减轻H_2O_2对海马CA1区细胞损伤,促进神经元突触传递功能的恢复,其作用机制与激活GABAA受体而减少细胞色素C的释放有关。
Objective: To study the protective effect of ginsenoside Rh2 on H 2 O 2 injury and its relationship with GABAA receptor. Methods: Rat hippocampal slices were prepared and divided randomly into normal group, model group, GABA group, ginsenoside Rh2 (20μmol / L, 40μmol / L, 60μmol / L), bicuculline group + Ginsenoside Rh2 group, each group 20 brain slices. The changes of cell viability in hippocampal CA1 region were detected by propidium iodide (PI) staining. The expression of cytochrome C was observed by immunohistochemistry. The synaptic transmission was observed by electrophysiological techniques. Results: Ginsenoside Rh2 (40μmol / L, 60μmol / L) significantly increased the viability of hippocampal CA1 region and decreased the expression of cytochrome C in hippocampal CA1 region. The recovery of OPS and the recovery of OPS in hippocampal slices were also significantly increased. The protective effect of ginsenoside Rh2 is similar to that of exogenous GABA and can be blocked by the GABAA receptor inhibitor bicuculline. CONCLUSION: Ginsenoside Rh2 can reduce the damage of H 2 O 2 to the hippocampal CA1 cell and promote the recovery of synaptic transmission in neurons. Its mechanism may be related to the activation of GABAA receptor and the decrease of cytochrome C release.