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目的:建立重庆地区HBV流行株PreS/S、EnhII/CP/PreC基因标准参照序列,为HBV变异的研究奠定基础。方法:测定15例无症状携带者HBV的PreS/S、EnhII/CP/PreC序列,应用同源性分析和对齐比较等方法确定基因型/血清型,并拟定标准参照序列。结果:9株基因型B/血清型;adw2、3株基因型B/M清型ayw1、3株基因型C/血清型adrq+;建立的重庆地区HBV流行株(基因型B/血清型adw2)PreS/S、EnhII/CP/PreC标准参照序列与华东华南地区同亚型的标准参照序列比较分别有6个、1个核苷酸差异。结论:初步建立了重庆地区HBV流行株PreS/S、EnhIICP/PreC基因标准参照序列。
OBJECTIVE: To establish a standard reference sequence of PreS / S and EnhII / CP / PreC genes in HBV epidemic strains in Chongqing, and lay the foundation for the study of HBV mutation. Methods: PreS / S and EnhII / CP / PreC sequences were determined in 15 asymptomatic HBV carriers. The genotypes and serotypes were determined by homology analysis and alignment comparison, and the standard reference sequences were prepared. Results: 9 strains of genotype B / serotypes; adw2,3 strains genotype B / M type ayw1,3 strains genotype C / serotypes adrq +; established Chongqing epidemic strains (genotype B / serotype adw2) The standard reference sequences of PreS / S and EnhII / CP / PreC were 6 and 1 nucleotide difference respectively compared with the standard reference sequences of isoforms in southern China. Conclusion: PreS / S and EnhIICP / PreC standard reference sequences were preliminarily established in Chongqing epidemic strains.