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为探索同步测定大鼠成骨细胞膜Ｃａ２＋－ＡＴＰ酶和Ｎａ＋、Ｋ＋－ＡＴＰ酶活性的方法。采用孔雀绿比色法测定磷以反映酶活性。结果显示：Ｃａ２＋浓度以及成骨细胞传代培养的代数是影响酶活性的重要因素。Ｃａ２＋有助于提高ＡＴＰａｓｅ活性。Ｃａ２＋浓度为４０μｍｏｌ／Ｌ时，ＡＴＰａｓｅ活性较高。细胞传代越多，膜Ｃａ２＋－ＡＴＰａｓｅ活性越低。成骨细胞膜Ｃａ２＋－ＡＴＰａｓｅ活性低于Ｎａ＋、Ｋ＋－ＡＴＰａｓｅ。结论：孔雀绿比色法同步测定大鼠成骨细胞膜Ｃａ２＋－ＡＴＰ酶和Ｎａ＋、Ｋ＋－ＡＴＰ酶活性，方法简便、灵敏、可靠。
To explore the simultaneous determination of rat osteoblasts membrane Ca2 + -ATP enzyme and Na +, K + -ATP enzyme activity method. Determination of phosphorus by malachite green colorimetric method to reflect enzyme activity. The results showed that Ca2 + concentration and algebra of subculturing osteoblasts were important factors affecting enzyme activity. Ca2 + helps to increase ATPase activity. When Ca2 + concentration was 40μmol / L, ATPase activity was higher. The more cells pass, the lower the membrane Ca2 + -ATPase activity. Osteoblast membrane Ca2 + -ATPase activity was lower than Na +, K + -ATPase. Conclusion: The activity of Ca2 + -ATPase and Na +, K + -ATPase in rat osteoblasts is determined synchronously by malachite green colorimetric assay. The method is simple, sensitive and reliable.