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为研究灰飞虱对噻嗪酮的抗性发展规律及抗性生化机理,采用稻苗喷雾法对灰飞虱种群进行连续筛选获得高抗性品系,估算其现实遗传力并进行田间抗性风险预测;采用稻苗浸渍法测定杀虫剂对灰飞虱的毒力及交互抗性;利用生物化学方法测定不同品系之间的解毒酶活力,探讨灰飞虱对噻嗪酮的抗性生化机理.结果表明:用噻嗪酮对灰飞虱种群连续筛选32代,其抗性倍数达到168.49倍,现实遗传力h~2为0.11.当杀死率为80%~90%时,预计灰飞虱对噻嗪酮的抗性增长10倍,仅需要5~6代.田间实际的现实遗传力要比室内选择种群估计低一些,预计田间抗性提高10倍所需要时间会更长.交互抗性测定结果表明,灰飞虱抗噻嗪酮品系与吡虫啉和噻虫嗪之间有高水平交互抗性,与啶虫脒有低水平交互抗性,与吡蚜酮和毒死蜱无交互抗性.增效作用和解毒酶活力测定结果显示,抗性品系细胞色素P450单加氧酶活力提高最大,酯酶次之,谷胱甘肽-S-转移酶无显著变化.田间使用噻嗪酮防治灰飞虱存在较大抗性风险,可与吡蚜酮和毒死蜱等交替使用以延缓抗性发展;3种解毒酶中,细胞色素P450单加氧酶在灰飞虱对噻嗪酮的抗性发展中起到了重要作用.
In order to study the resistance development and resistance biochemical mechanism of Laodelphax striatellus to buprofezin, the rice planthopper population was screened continuously to obtain highly resistant strains, and the actual heritability and field resistance risk The bioassay was used to determine the toxicity and interactive resistance of the insecticides against Laodelphax striatellus (L.) The results showed that the population of Laodelphax striatellus was continuously screened for 32 generations with buprofezin, the resistance multiples reached 168.49 times, and the actual heritability h ~ 2 was 0.11. When the killing rate was 80% ~ 90% Louse resistance to buprofezin tenfold increased, only 5 to 6 generations, and the actual actual heritability in the field was estimated to be lower than that for indoor selection, and the time required to increase resistance tenfold in the field would be longer. The results of sex determination indicated that there was a high level of cross resistance between the Bt strain against imidacloprid and thiamethoxam, low level of cross resistance to acetamiprid and no cross-resistance to pymetrozine and chlorpyrifos. Synergism and detoxification enzyme activity assay results show that the resistant strain cytochrome P450 Oxygenase activity increased the most, followed by esterase, glutathione-S-transferase no significant changes in field use of buprofezin against the SBH there is a greater resistance risk, with pymetrozine and chlorpyrifos and other alternate Was used to delay the development of resistance. Of the three detoxification enzymes, cytochrome P450 monooxygenase played an important role in the resistance development of buprofezin to buprofezin.