Optimization of mapping rule of bit-interleaved Turbo coded modulation with 16 quadrature amplitude modulation (QAM) is investigated based on different impacts of various encoded bits sequence on Turbo decoding performance. Furthermore, bit-interleaved in-phase and quadrature phase (I-Q) Turbo coded modulation scheme are designed similarly with I-Q trellis coded modulation (TCM). Through performance evaluation and analysis, it can be seen that the novel mapping rule outperforms traditional one and the I-Q Turbo coded modulation can not achieve good performance as expected. Therefore, there is not obvious advantage in using I-Q method in bit-interleaved Turbo coded modulation.