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目的研究环境噪音对新生大鼠身长体质量及学习记忆的影响。方法选用7 d SD大鼠16只,分为高噪音组和对照组,每组8只,高噪音组的平均噪音为90 db(A)、对照组50 db(A)。分别在P10、P15、P20时进行身长、体质量的测量,P20时利用Morris水迷宫观察学习记忆功能、ELISA检测生长激素,RT-PCR、Western blot观察突触素表达。结果高噪音组身长、体质量低于同年龄对照组大鼠[身长(cm):P10(9.54±0.1)vs(9.98±0.1),P15(10.24±0.1)vs(10.79±0.1),P20(10.59±0.1)vs(11.68±0.1);体质量(g):P10(14.33±0.02)vs(14.51±0.02),P15(15.31±0.02)vs(15.68±0.02),P20(16.63±0.02)vs(16.76±0.02),P<0.05];与对照组比较,高噪音组学习能力下降,生长激素、突触素(synaptophysin,SYP)mRNA、SYP表达水平低于同年龄对照组大鼠[生长激素(pg/ml):(299.7±4.9)vs(504.9±13.14),SYP mRNA:(0.182±0.008)vs(1.056±0.011),SYP:(0.168±0.007)vs(0.432±0.025),P<0.05]。结论高噪音环境下新生SD大鼠的身长、体质量增长缓慢,学习记忆能力下降。
Objective To study the effects of environmental noise on body weight and learning and memory of newborn rats. Methods Sixteen SD rats aged 7 days were divided into high noise group and control group with 8 rats in each group. The average noise of high noise group was 90 db (A) and that of control group was 50 db (A). Body length and body weight were measured at P10, P15 and P20 respectively. Morris water maze was used to observe the learning and memory function at P20. Growth hormone was detected by ELISA. The expression of synaptophysin was detected by RT-PCR and Western blot. Results The body weight of high noise group was lower than that of the same age group [height (cm): P10 (9.54 ± 0.1) vs (9.98 ± 0.1), P15 (10.24 ± 0.1) vs (10.79 ± 0.1) (P <0.05); P <0.05); P <0.05); P <0.05) (16.76 ± 0.02), P <0.05] .Compared with the control group, the learning ability decreased in high noise group and the mRNA and protein expression of growth hormone, synaptophysin (SYP) and SYP were lower than those in the control group [growth hormone (pg / ml) :( 299.7 ± 4.9) vs (504.9 ± 13.14), SYP mRNA: (0.182 ± 0.008) vs (1.056 ± 0.011), SYP: (0.168 ± 0.007) vs (0.432 ± 0.025) ]. Conclusion The body length and body weight of neonatal SD rats grow slowly and the learning and memory abilities decrease in high-noise environment.