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给肝癌大鼠注射济南假单胞菌苗,连续12周.分别于第8、12、16周从同只大鼠体内分离出肝枯否细胞,腹腔巨噬细胞,肺巨噬细胞和血液单核细胞.并分别与人肝癌细胞联合培养,测定5种效应细胞对肝癌细胞的抑增殖作用.结果显示:5种效应细胞对肝癌细胞均具有自然增殖作用,作用强度依次为枯否细胞、腹腔巨噬细胞、脾巨噬细胞、血液单核细胞.在体应用济南假单胞菌苗后,5种效应细胞的抑肝癌细胞作用均显著增强,抑制率分别提高了59.2%～174.2%不等.在体实验为临床肝癌免疫治疗应用济南假单胞菌苗的可能性提供了实验依据.
Hepatoma cells were injected with Pseudomonas syphilis vaccine for 12 consecutive weeks. Liver Kupffer cells, peritoneal macrophages, pulmonary macrophages and blood cells were isolated from the same rats on the 8th, 12th, and 16th weeks respectively. The nucleus cells were co-cultured with human hepatoma cells, and the effect of five effector cells on the proliferation of hepatocellular carcinoma cells was determined. The results showed that: 5 kinds of effector cells have a natural proliferation effect on hepatoma cells, the order of magnitude is Kupffer cells, abdominal cavity Macrophages, splenic macrophages, and blood mononuclear cells. After the application of Pseudomonas syphilis in Jinan, the effects of the five effector cells on the inhibition of hepatoma cells were significantly enhanced, and the inhibition rates increased by 59.2% to 174.2%, respectively. In vivo experiments provide experimental basis for the possibility of clinical application of hepatocarcinoma immunotherapy to pseudomonas vaccination in Jinan.