论文部分内容阅读
为了解Cu/Zn超氧化物歧化酶分子伴侣基因(CCS)在结缕草逆境胁迫过程中的表达调控机制,以结缕草(Zoysia japonica)盐胁迫条件下的转录组数据为依据,结缕草cDNA为模板,通过RT-PCR法,克隆了924bp长的结缕草ZjCCS基因的CDS序列。同源性分析显示该基因与谷子(Setaria italica)同源性最高,含有植物CCS蛋白所特有的3个保守结构域。利用实时荧光定量PCR(qRT-PCR)技术研究该基因在非生物胁迫条件下在结缕草叶片中的表达情况,结果显示,在盐胁迫条件下,ZjCCS基因的表达量呈先上升后下降的趋势,4h表达量最高,相对于盐胁迫,干旱和重金属胁迫对ZjCCS基因的表达量影响不大,推测ZjCCS基因可能在结缕草响应盐胁迫过程中发挥作用。
In order to understand the mechanism of Cu / Zn superoxide dismutase (SOD) chaperone gene (CCS) expression during the stress of Zoysia matrella, and based on the transcriptome data of Zoysia japonica under salt stress conditions, Grass cDNA was used as a template to clone the CDS sequence of ZjCCS gene of 924bp long Zoysiagrass by RT-PCR. Homology analysis showed that this gene has the highest homology with Setaria italica and contains 3 conserved domains unique to plant CCS protein. The qRT-PCR technique was used to study the expression of ZjCCS in the leaves of Zoysiagrass under abiotic stress conditions. The results showed that under salt stress, the expression of ZjCCS firstly increased and then decreased The trend was highest at 4h. Compared with salt stress, drought and heavy metal stress had little effect on the expression of ZjCCS gene, suggesting that ZjCCS may play a role in salt stress response to Zoysia japonica.