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利用涂布平板法从腐烂秸秆及枯枝烂叶中分离出71株丝状真菌.通过比较菌株秸秆粉降解产物对小麦生长的促进作用,获得一株可有效降解秸秆并促进小麦生长的菌株2-5-2.对其进行形态分析和rRNA序列鉴定,结果显示,该菌株为木霉属真菌.该菌株可产生纤维素酶、木质素酶、半纤维素酶、几丁质酶、蛋白酶、壳聚糖酶.平板对峙培养显示,该菌对多种病原真菌的生长有抑制或阻碍作用.固态发酵培养时,纤维素酶、半纤维素酶酶活在第11d达到最高;第28d,纤维素降解率达到21.13%,半纤维素降解率达到28.53%.
71 strains of filamentous fungi were isolated from rotten straw and rotten leaves by coating method.The strain 2 -5-2. Morphological analysis and rRNA sequence identification showed that the strain was Trichoderma fungi, which could produce cellulase, ligninase, hemicellulase, chitinase, protease, Chitosanase and plate confrontation culture showed that this strain could inhibit or impede the growth of many kinds of pathogenic fungi.Cellulase and hemicellulase activity reached the highest on the 11th day in the solid state fermentation culture.At the 28th day, The degradation rate reached 21.13% and the degradation rate of hemicellulose reached 28.53%.