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目的总结工作经验,与广大预防医学工作者进行交流,加强流感监测工作,提高监测水平,更好地为流感防控工作服务。方法从标本的采集、处理、保存、实验操作中的细节处、生物安全等多方面着手,分析利弊,总结经验,提出注意事项。结果实验前严格筛选标本,定期核查仪器设备,实验中提高胰酶浓度缩短消化细胞时间,提高病毒感染温度缩短感染时间,收获病毒时反复冻融,血凝和血凝抑制实验使用豚鼠血红血球,借助其他实验方法辅助检测都能提高流感病毒的检出率。结论随着全国流感监测网络实验室的普及,从事流感监测工作的预防医学工作者越来越重视实验室流感病毒检出率的提高,通过总结交流工作经验,对影响流感病毒组织培养实验中的关键点加以控制,能有效提高流感病毒的检出率。
Objective To summarize the work experience, communicate with the general preventive medicine workers, strengthen the work of influenza surveillance, improve the monitoring level and better serve the prevention and control of influenza. Methods From the aspects of collection, processing, preservation, details of experiment, biosafety and so on, the advantages and disadvantages were analyzed, the experiences were summarized and the precautions were given. Results Strict screening of specimens before the experiment, regular inspection equipment, experimental increase trypsin concentration to shorten the digestive cell time, increase the temperature of the virus infection to shorten the infection time, the virus repeatedly freeze-thaw, coagulation and hemagglutination inhibition experiments using guinea pig blood red blood cells, Assisted testing with other experimental methods can improve the detection rate of influenza virus. Conclusion With the popularization of the National Influenza Surveillance Network Laboratory, prevention medical workers engaged in influenza surveillance are paying more and more attention to the increase of the detection rate of laboratory influenza viruses. By summarizing the exchange work experience, Key points to be controlled, can effectively improve the detection rate of influenza virus.