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目的建立高效无毒TQ纯天然植物蚊虫驱避剂的质量控制标准,研究TQ纯天然植物蚊虫驱避剂的驱蚊效果。方法选用川芎、姜黄、羌活、蛇床子、桉叶、薄荷6种药材的挥发油复方制备TQ纯天然植物蚊虫驱避剂,采用薄层色谱法对制剂中的6种成分进行定性鉴别;采用高效液相色谱法测定制剂中的藁本内酯含量;采用气相色谱法测定制剂中的薄荷脑含量;根据国标研究TQ纯天然植物蚊虫驱避剂的驱蚊作用。结果薄层色谱法可鉴别6种成分,斑点清晰,分离度好;高效液相色谱法测定藁本内酯,在1.08~6.48μg范围呈现良好的线性关系(r=0.999 6),平均回收率为99.12%,TQ蚊虫驱避剂含藁本内酯≥19.80 mg/ml;气相色谱法测定薄荷脑,在0.175~0.875μg范围呈现良好的线性关系(r=0.999),平均回收率为99.95%,TQ蚊虫驱避剂含薄荷脑不得少于6.10 mg/ml;白纹伊蚊攻击力实验合格,TQ纯天然植物蚊虫驱避剂有效保护时间>6 h。结论制定的驱避剂质量标准操作简便易行、准确可靠、灵敏度高、重现性好,可作为TQ纯天然植物蚊虫驱避剂的质量控制方法。TQ纯天然植物蚊虫驱避剂有效保护时间>6 h,达国家驱避剂登记的A级标准。
Objective To establish a quality control standard for efficient and nontoxic TQ natural plant mosquito repellent and to study the mosquito repellent effect of TQ natural plant mosquito repellent. Methods TQ natural plant mosquito repellent was prepared by using the volatile oil of six herbs of Rhizoma Chuanxiong, Turmeric, Notopterygium, Cnidium, Eucalyptus and Mint. The six components in the preparation were qualitatively identified by TLC. Determination of ligustilide content in the preparation by chromatographic method; menthol content in the preparation by gas chromatography; repellent effect of TQ natural plant mosquito repellent according to the national standard. Results Six kinds of components were identified by TLC, the spots were clear and the resolution was good. The determination of ligustilide by HPLC showed a good linear relationship in the range of 1.08 ~ 6.48 μg (r = 0.999 6), the average recovery 99.12%, TQ mosquito repellent containing ligustilide ≥19.80 mg / ml; gas chromatographic determination of menthol showed a good linearity (r = 0.999) in the range of 0.175 ~ 0.875 μg with an average recovery of 99.95% , TQ mosquito repellent containing menthol not less than 6.10 mg / ml; Aedes albopictus attack qualified, TQ natural plant mosquito repellent effective protection time> 6 h. Conclusions The repellent quality standards developed are easy to operate, accurate and reliable, high sensitivity and good reproducibility, and can be used as quality control methods for natural plant repellent of TQ. TQ natural plant mosquito repellent effective protection time> 6 h, up to the national repellent registration level A standard.