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目的:评价组织隔离法与机械活动法在建立兔胫骨萎缩型与肥大型骨不连模型中的作用。方法:将12只体质量3～4.5kg的新西兰大白兔随机分成A、B2组,A组于胫骨中段截骨,两断端套接1cm硅胶管,单侧外固定器固定,保持两断端间距2mm,8周取出硅胶管,观察组织隔离法构建萎缩型骨不连模型的效果。B组于胫骨中段截骨后采用2枚1mm克氏针行髓内松动固定,被动活动断端200次/d,持续1个月,观察机械活动法构建肥大型骨不连模型的效果。结果:A组中所有动物在硅胶管取出后4周,无1例断端出现骨愈合表现,X线片显示良好萎缩型骨不连的复制。B组中所有动物在6周内截骨端出现延迟愈合,部分伴有畸形,骨断端有大量肥大骨痂形成。结论:硅胶管组织隔离法是复制兔胫骨萎缩型骨不连模型的有效方法,而采用被动机械活动复制兔胫骨肥大型骨不连模型的方法尚需进一步研究。
OBJECTIVE: To evaluate the role of tissue isolation and mechanical activity in establishing a model of tibial atrophy and hypertrophic nonunion in rabbits. Methods: Twelve New Zealand white rabbits with body weight of 3 ~ 4.5kg were randomly divided into group A and group B. Group A was osteotomied in the middle of the tibia. The two ends were fixed with a 1cm silicone tube and fixed with one lateral external fixator. Pitch 2mm, 8 weeks removed the silicone tube to observe the effect of tissue isolation method to construct atrophic nonunion model. Group B received osteotomy in the middle of the tibia. Two 1mm Kirschner wires were used for intramedullary loosening. The passive ends were doused for 200 times per day for one month. The effect of mechanical mobilization on the formation of hypertrophic nonunion model was observed. Results: All the animals in group A showed no signs of bone healing 4 weeks after silicone tube was removed, and X - ray showed good atrophic nonunion. In group B, all the animals appeared delayed healing within 6 weeks, some were accompanied by deformity, and a large number of hypertrophic callus formed at the fracture site. Conclusion: Silicone tube tissue isolation is an effective method to replicate rabbit atrophic nonunion model of tibia. However, the method of passive mechanical replication of rabbit tibial hypertrophic nonunion model remains to be further studied.