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腹水和胸水中脱落细胞的萤光显微镜检查。李文镇等(河北医学院)。天津医药杂志7:510,1965。 Wittkind(1958)倡用本法。Bertalanffy(1962)用本法观察胸、腹水624例。作者取新鲜胸腹水,离心沉淀涂片,95％酒精固定10分钟后Bertalanffy染色并用巴氏染色对照。炎细胞:多核白血球和淋巴球,胞核呈绿色,胞浆浅绿或橙色,吞噬细胞核浅黄,结构清楚,浆细胞萤光强而浓密。活跃的间皮细胞胞体较小,萤光强而浓密,核色黄或黄绿,偶有
Fluorescence microscopy of exfoliated cells in ascites and pleural fluid. Li Wenzheng et al. (Hebei Medical College). Tianjin Medical Journal 7:510,1965. Wittkind (1958) advocated this method. Bertalanffy (1962) used this method to observe 624 cases of chest and ascites. The authors took fresh pleural effusion and ascites fluid and centrifuged the smears. After 95% alcohol was fixed for 10 minutes, they were stained with Bertalanffy and stained with Pap for control. Inflammatory cells: polynuclear leukocytes and lymphocytes, with green nucleus, pale green or orange cytoplasm, phagocytic nucleus light yellow, well-structured, and dense and dense plasma cells. Active mesothelial cell body is small, strong fluorescent and dense, nuclear yellow or yellowish green, occasionally