目的通过对感染蚤死亡后拉胃培养及蚤的粪便培养,研究人蚤带菌及蚤粪便带菌时限。方法用50亿个鼠疫菌∕m L的人全血饲养人蚤3d,镜下观察吸血情况,取吸血蚤拉胃培养,计算感染率,剩下蚤用人全血进行群体健康饲养,每天观察并换血,死蚤拉胃培养,用白色消毒滤纸采集粪便,每天更换1次,培养1次,每次培养8份,一直到蚤死完为止。结果试验蚤鼠疫菌感染率91.7%;80份蚤粪便培养,28份阳性(阳性率35%),蚤粪便带菌9d;106匹蚤死亡后拉胃培养,38匹阳性(阳性率35.85%),感染蚤最短存活1d,最长12d,平均4.5d,最长带菌12d。结论人蚤感染鼠疫菌后寿命较短,明显影响通过叮咬传播鼠疫的媒介地位;人蚤粪便带菌9d,根据其生物学特性可能会造成非菌栓形式传播,因此人蚤粪便带菌具有重要的流行病学意义。 OBJECTIVE To study the culture of fleas and flea stool by studying the culture of rabbits infected with fleas and the stool culture of fleas. Methods Human fleas 3d were fed with 5 billion Yersinia pestis / m L whole blood. The blood samples were taken for microscopic observation. The infection rate was calculated by taking the stomach of sucking blood fleas, and the remaining fleas were kept in groups with human whole blood. Transfusion, dead fleas pulled stomach culture, white fecal filter paper collection, changing 1 times a day, training 1, each training 8, until the dead fleas date. The results showed that the infection rate of fleas was 91.7%, 80 flea stool cultures were positive (positive rate was 35%) and flea stool was 9d. After fleas death, 106 rabbits were cultured in the lavage, 38 (positive rate 35.85%), Infected fleas minimum survival 1d, the longest 12d, an average of 4.5d, the longest carriage 12d. Conclusion Human fleas infected with Yersinia pestis have shorter lifespan and significantly affect the vector status of plague-transmitted plague. Human flea faecal carrier 9d, which may be caused by non-sectarian transmission according to its biological characteristics, is therefore an important epidemic Meaning of the disease.