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鼻咽癌病毒病因的研究是在大量血清流行病学的基础上,建立分子生物学手段检查癌上皮细胞中的EB病毒基因,用以阐明鼻咽癌与EB病毒的关系:(1)除人的B淋巴细胞外,在其他类型的细胞中EB病毒均不能复制,带EB病毒的淋巴母细胞株也只产生极少的病毒颗粒;(2)从病毒感染的细胞中制备病毒核酸,不可避免地会污染少量细胞DNA,而直接影响了反应的特异性,因此迫切需要用基因工程手段制备EB病毒核酸;(3)直接研究癌组织中的EB病毒核酸,需要更敏感而有效的探针,以用于原位核酸杂交技
The etiology of nasopharyngeal carcinoma virus is based on a large number of serum epidemiology, the establishment of molecular biology tools to examine the EB virus gene in cancer epithelial cells to clarify the relationship between nasopharyngeal carcinoma and Epstein-Barr virus: (1) In addition to human Of the B lymphocytes in the other types of cells in the EB virus can not be copied, with Epstein-Barr virus lymphoblastoid cell line produces only very few virus particles; (2) virus-infected cells prepared from the virus nucleic acid is inevitable Therefore, it is urgent to use genetic engineering methods to prepare Epstein-Barr virus nucleic acid. (3) To directly study Epstein-Barr virus nucleic acid in cancer tissue, more sensitive and effective probes are needed, For in situ nucleic acid hybridization