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目的:研究临床分离医院感染鲍曼不动杆菌中Ⅰ类整合子的分布及其各菌株间的同源性。方法:以特异性引物分别扩增Ⅰ类整合酶基因和Ⅰ类整合子全基因组,以确定其在鲍曼不动杆菌中的分布,对整合子基因盒进行克隆测序,了解其携带的耐药信息;应用RAPD技术对其进行基因分型,绘制亲缘关系树状图,分析菌株间的同源性。结果:19株医院感染鲍曼不动杆菌中16株携带Ⅰ类整合酶基因,8株携带Ⅰ类整合子基因盒。RAPD技术将其分为4个基因型,其中主要为B型14株,占73.7%,其次为A型3株,C型和D型各1株。C型与B型亲缘关系最近,两者有66%的同源性。结论:医院感染鲍曼不动杆菌中Ⅰ类整合酶的携带率高达84.2%,是鲍曼不动杆菌多药耐药迅速发生的一个重要原因;同源性分析确定我院急诊ICU和住院ICU之间存在多药耐药鲍曼不动杆菌的院内克隆传播。
Objective: To study the distribution of class Ⅰ integrons in Acinetobacter baumannii isolates from clinically isolated hospitals and their homology among isolates. Methods: The whole genome of class Ⅰ integrase gene and class Ⅰ integrase were amplified by specific primers to determine their distribution in Acinetobacter baumannii. The integron gene cassette was cloned and sequenced to find out the resistance RAPD technique was used to genotype them and the phylogenetic tree was drawn to analyze the homology among isolates. Results: Of 19 isolates of Acinetobacter baumannii, 16 strains carried type Ⅰ integrase gene and 8 strains carried type Ⅰ integron gene cassette. RAPD technology divides it into 4 genotypes, of which 14 are B type, accounting for 73.7%, followed by 3 types of A type and 1 type of C type and D type respectively. The closest relationship between type C and type B, the two have 66% homology. Conclusion: The prevalence of type Ⅰ integrase in Acinetobacter baumannii infection is as high as 84.2%, which is an important reason for the rapid development of multidrug-resistant Acinetobacter baumannii. Homology analysis identified the ICU and hospital ICU Between the existence of multidrug-resistant Acinetobacter baumannii hospital cloning.