甲基化糖的分析广泛地用于研究寡糖和多糖分子中单糖残基的键合位置及各种键合位置的相对数量。实现这种研究的可靠手段是气相色谱-质谱仪。与气相色谱法相比较,高效液相色谱法的优点是样品无需乙酰基化处理,同时又适于分析各种糖类及衍生物。但液相色谱法存在两个困难:甲基化糖的分析需用示差折光检测器,因此,无法采用梯度洗脱法分离保留时间 The analysis of methylated sugars is widely used to study the relative number of bonded sites and various bonded sites of monosaccharide residues in oligosaccharides and polysaccharide molecules. A reliable means of achieving this is GC-MS. Compared with gas chromatography, HPLC has the advantage of the sample without acetylation, but also suitable for the analysis of various sugars and derivatives. However, there are two difficulties with liquid chromatography: the analysis of methylated sugars requires a refractive index detector and, therefore, the retention time can not be separated by gradient elution