[Objective] To establish a quantitative method for the determination of total flavonoids from different medicinal parts in Rhamnus songorica L, which was a Xinjiang Kazakh traditional medicine. [Method] Flavonids in different parts of R. songorica were extracted with 70% ethanol as the ultrasound solvent. The total flavonoids were determined by spectrophotometry at 510 nm with rutin as the control. [Result] The absorbency and concentration of rutin had good linear correlation in the range of 8-40 mg/L. The regression equation was Y=9.721 4X+0.000 6 (R=0.999 8). The average recovery rates (branches, leaves and fruits of R. songorica) were 100.3%, 99.2% and 100.0%, with RSD being 1.58%, 1.87% and 1.70%, respectively. [Conclusion] The method was simple, accurate and reliable. It could be used to detect the content of total flavonoids in different medicinal parts of R. songorica. [Objective] To establish a quantitative method for the determination of total flavonoids from different medicinal parts in Rhamnus songorica L, which was a Xinjiang Kazakh traditional medicine. [Method] Flavonids in different parts of R. songorica were extracted with 70% ethanol as the ultrasound solvent. The total flavonoids were determined by spectrophotometry at 510 nm with rutin as the control. [Result] The absorbency and concentration of rutin had good linear correlation in the range of 8-40 mg / L. The regression equation was Y = 9.721 4X + 0.000 6 (R = 0.999 8). The average recovery rates (branches, leaves and fruits of R. songorica) were 100.3%, 99.2% and 100.0%, with RSD being 1.58%, 1.87% and 1.70%, respectively. [Conclusion] The method was simple, accurate and reliable. It could be used to detect the content of total flavonoids in different medicinal parts of R. songorica.