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目的观察单次大剂量碘对大鼠甲状腺内钠碘转运体(NIS)蛋白表达量的影响,探讨应用99mTcO-4进行甲状腺扫描前是否应对碘的摄入进行限制。方法将SD大鼠分成给碘后1d处死组、给碘后7d处死组和对照组,每组8只;将2000μg碘化钠(NaI)溶于0.5ml生理盐水注入前两组大鼠腹腔内,对照组注射等量生理盐水。各组大鼠处死后,取其甲状腺,应用Western免疫印迹方法探测NIS蛋白含量,通过光密度分析方法对信号强度进行半定量分析。结果给碘后1d处死组大鼠甲状腺内NIS蛋白含量明显低于对照组和给碘后7d处死组(P均<0.001),而给碘后7d处死组与对照组间NIS蛋白表达量的差异无统计学意义(P>0.05)。结论单次大剂量碘摄入可降低甲状腺内NIS蛋白的表达量,此效应在给碘1d后明显,7d后可恢复至正常水平。
Objective To observe the effect of single high-dose iodine on the expression of sodium iodide transporter (NIS) in rat thyroid gland, and to investigate whether iodine uptake should be limited before 99mTcO-4 thyroid scan. Methods The SD rats were divided into sacrificial group 1 d after iodine injection, sacrificed 7 d after iodine injection and control group (n = 8 each). 2000 μg sodium iodide (NaI) dissolved in 0.5 ml normal saline was injected into the abdominal cavity The control group received the same amount of saline. After the rats were sacrificed, the thyroid gland was taken and the Western blotting was used to detect the content of NIS protein. The signal intensity was semi-quantitatively analyzed by optical density analysis. Results The content of NIS protein in the thyroid of rats sacrificed at 1 day after iodine treatment was significantly lower than that of the control group and sacrificed at 7 days after iodine treatment (P <0.001), while the differences of NIS protein expression between the sacrificed group and the control group No statistical significance (P> 0.05). Conclusion Single dose of iodine intake can reduce the expression of NIS protein in the thyroid gland. This effect is obvious on the 1st day of iodine treatment and returns to the normal level after 7 days.