目的：在体外研究平阳霉素（ＰＹＭ）对人脐静脉血管内皮细胞（ＨＵＶＥＣ）的作用。方法：在体外培养的ＨＵＶＥＣ中加入不同浓度的ＰＹＭ，培养０．５ｈ及１ｈ后取贴壁细胞及培养液进行研究。结果：⑴作用１ｈ后，２５μｇ·ｍｌ１以上浓度组均可见细胞多数收缩，随浓度增高，细胞脱落增多。⑵随药物浓度、作用时间增加，培养液中ＬＤＨ活性及细胞杀伤率明显升高。结论：ＰＹＭ对ＨＵＶＥＣ损伤具有剂量和时间依赖性。防止局部注射药物流失可能是治疗海绵状血管瘤的关键。 Objective: To study the effect of PYM on human umbilical vein endothelial cells (HUVECs) in vitro. Methods: Different concentrations of PYM were added to HUVEC cultured in vitro, and cultured in vitro for 0.5h and 1h. Results: (1) After 1 h of treatment, most of the cells showed shrinkage at the concentration of 25μg · ml1 or above. With the increase of the concentration, the cells shedding increased. ⑵ with the drug concentration, the role of time increased, LDH activity in culture medium and cell killing significantly increased. Conclusion: PYM has dose-and time-dependent effect on HUVEC injury. Prevention of local injection of drug may be the key to the treatment of cavernous hemangiomas.