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建立了快速测定苯巴比妥含量的方法。在pH 6.0的Clark-Lubs(C-L)缓冲溶液中,向一定浓度十六烷基三甲基溴化铵中加入荧光桃红,体系荧光增强,再加入苯巴比妥,体系荧光强度降低,且药品加入量与降低程度呈良好线性关系。在优化试验的条件下,线性范围是0.008~0.72 mg/L,检出限为0.02 mg/L。由该体系检测了药品在动物血清中的含量,回收率为93.6%~97.3%,相对标准偏差在2.3%~3.2%之间。建立了荧光桃红-十六烷基三甲基溴化铵测定苯巴比妥含量的荧光探针法。
A rapid method for the determination of phenobarbital was established. Fluorescent pink was added to cetyltrimethylammonium bromide at a certain concentration in Clark-Lubs (CL) buffer solution at pH 6.0. The fluorescence of the system was enhanced and then phenobarbital was added. The fluorescence intensity of the system decreased. There is a good linear relationship between adding amount and reducing degree. Under the optimized conditions, the linear range was 0.008 ~ 0.72 mg / L with the detection limit of 0.02 mg / L. The content of the drug in animal serum was detected by this system. The recoveries ranged from 93.6% to 97.3% with relative standard deviations between 2.3% and 3.2%. A fluorescent probe method for the determination of phenobarbital with fluorescent pink-cetyltrimethylammonium bromide was established.