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目的:观察益气温阳护卫汤对哮喘豚鼠气道嗜酸性粒细胞浸润及活化的影响。方法:将豚鼠随机分为正常对照组、模型组、地塞米松组、玉屏风散组及益气温阳护卫汤低、中、高剂量组。于诱喘后24 h,采集支气管肺泡灌洗液(BALF)进行嗜酸性粒细胞(EOS)直接计数、细胞分类计数;检测 BALF 中嗜酸性粒细胞阳离子蛋白(ECP)的含量;并测定支气管粘膜及粘膜下 EOS 数目。结果:益气温阳护卫汤各剂量组 BALFEOS 数均低于哮喘组(P<0.05);中剂量组、高剂量组与地塞米松组三者之间差异无显著性(P>0.05)。高剂量组支气管粘膜及粘膜下 EOS 计数与地塞米松组比较差异无显著性(P>0.05),与其他组比较差异有显著性(P<0.05)。中剂量组、高剂量组与地塞米松组三者之间 BALF ECP 含量无明显差异(P>0.05),与其他组比较差异有显著性(P<0.05)。结论:益气温阳护卫汤可以减轻哮喘豚鼠气道 EOS 浸润及活化,故可用于哮喘的防治。
Objective: To observe the effect of Yiwen Yangyang Huwei Decoction on the infiltration and activation of eosinophils in asthmatic guinea pig airways. Methods: The guinea pigs were randomly divided into normal control group, model group, dexamethasone group, Yupingfengsan group and low-, medium-, and high-dose groups of Yiwenyangyang Huwei Tang. 24 hours after asthma induction, bronchoalveolar lavage fluid (BALF) was collected for direct eosinophil (EOS) counts, cell differential counts, eosinophil cationic protein (ECP) levels in BALF, and bronchial mucosa. And submucosal EOS number. RESULTS: The BALFEOS was lower in each dose group than in the asthma group (P<0.05). There was no significant difference between the middle dose group, high dose group, and dexamethasone group (P>0.05). There was no significant difference in bronchial mucosa and submucosal EOS count between the high-dose group and the dexamethasone group (P>0.05), and there was a significant difference compared with other groups (P<0.05). There was no significant difference in BALF ECP content between the middle dose group, high dose group and dexamethasone group (P>0.05), and there was a significant difference compared with other groups (P<0.05). Conclusion: Yi-Wan-Yang-Yang-Wei-Wei-Tang can reduce the infiltration and activation of airway EOS in asthmatic guinea pigs, so it can be used for the prevention and treatment of asthma.