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目的:建立一种流动注射-化学发光测定利福平的快速、灵敏新方法。方法:碱性条件下,利福平对luminol-KIO4化学发光体系有抑制作用,结合流动注射技术,建立了流动注射-化学发光检测利福平的新方法。结果:在优化条件下,利福平的线性范围是7.0×10-9～6.0×10-7 mol.L-1,检出限达3.2×10-9 mol.L-1。对3.0×10-8 mol.L-1和2.0×10-7mol.L-1利福平分别进行11次测定,相对标准偏差分别为0.8%和1.6%。分析速度为128个样品/时。将提出的新方法成功应用于利福平滴眼液和利福平胶囊中利福平含量的测定。结论:本方法操作简单快速,适合于利福平的分析。
Objective: To establish a rapid and sensitive new method for the determination of rifampin by flow injection-chemiluminescence. METHODS: Rifampicin inhibited the luminol-KIO4 chemiluminescence system under alkaline conditions. A new method for the determination of rifampin by flow injection-chemiluminescence was established by flow injection technique. Results: Under optimized conditions, the linear range of rifampicin was 7.0 × 10-9 ~ 6.0 × 10-7 mol·L-1 with a detection limit of 3.2 × 10-9 mol·L-1. The determination of 3.0 × 10-8 mol·L-1 and 2.0 × 10-7 mol·L-1 rifampicin were carried out 11 times with relative standard deviations of 0.8% and 1.6% respectively. Analysis speed is 128 samples / hour. The proposed new method was successfully applied to the determination of rifampicin in rifampicin eye drops and rifampicin capsules. Conclusion: The method is simple and fast, suitable for rifampin analysis.