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目的:观察逆转录酶抑制剂叠氮胸苷(齐多夫定,Azidothymidine,AZT)联合放射线对人喉鳞癌Hep-2细胞裸鼠皮下移植瘤的治疗作用。方法:建立人喉鳞癌荷瘤裸鼠模型,应用AZT联合放射线治疗人喉鳞癌裸鼠移植瘤,观察其对肿瘤体积、端粒酶活性、hTERT蛋白表达效率及凋亡的影响。结果:与对照组相比,放射组和AZT+放射组肿瘤生长速度受到明显抑制(P<0.05),提示放疗、AZT联合放疗均能抑制肿瘤生长,且后者抑瘤率显著高于前者(P<0.05)。端粒酶活性(TA)及hTERT蛋白检测结果一致,即放射组>对照组>AZT+放射组>AZT组,提示低剂量放疗能够引起肿瘤细胞端粒酶活性的升高,而AZT能够有效抑制抑制辐射所致的端粒酶活性增高。凋亡指数为AZT+放射组(9.60±0.97%)>单纯放射组(6.91±1.65%)>AZT组(5.17±0.71%)>对照组(0.77±0.27%)。结论:AZT联合放射线能有效的抑制人喉癌裸鼠移植瘤的生长,AZT能增加肿瘤的放射敏感性。
Objective: To observe the therapeutic effect of reverse transcriptase inhibitor Azidothymidine (AZT) combined with radiation on subcutaneous xenografts of human laryngeal squamous cell carcinoma Hep-2 cells in nude mice. METHODS: Human laryngeal squamous cell carcinoma (HCC) tumor-bearing nude mice model was established. The transplanted tumor of human laryngeal squamous cell carcinoma in nude mice was treated with AZT combined with radiation. The effect of tumor volume, telomerase activity, hTERT protein expression and apoptosis were observed. Results: Compared with the control group, the tumor growth rate of radiotherapy group and AZT + radiotherapy group was significantly inhibited (P <0.05), suggesting that radiotherapy and AZT combined with radiotherapy can inhibit tumor growth, and the latter tumor inhibition rate was significantly higher than the former (P <0.05). Radiation group> control group> AZT + radiation group> AZT group, suggesting that low dose radiation therapy can cause the telomerase activity of tumor cells to rise, while AZT can effectively inhibit the inhibition Radiation-induced telomerase activity increased. The apoptotic index was (9.60 ± 0.97%) in AZT + radiotherapy group (6.91 ± 1.65%) vs 5.17 ± 0.71% in AZT group (0.77 ± 0.27%). Conclusion: AZT combined with radiation can effectively inhibit the growth of human laryngeal carcinoma xenografts in nude mice, and AZT can increase the radiosensitivity of tumors.