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目的探讨慢性乙型肝炎患者外周血来源树突状细胞(Dendriticcell,DC)数量及表型的改变,并对其与肝功能、乙肝病毒复制水平的关系进行研究。方法检测37例慢性乙肝患者和21例健康人肝功能及血清HBVDNA水平,并提取外周血单个核细胞(Peripheralbloodmononuclearcell,BMC)进行体外诱导培养,促使其发育成DC,计数其数量并检测膜表面分子的变化。分析DC数量及表型与肝功能、乙肝病毒复制水平的关系。结果与正常对照组比较,慢性乙肝患者的树突状细胞数量明显减少(P<0.05),且其膜表面分子CD83、CD86的表达均明显降低(P<0.05)。在慢性乙肝患者中,DC数量、DC膜表面分子CD83和CD86与血清HBVDNA之间呈负相关关系,而与肝功能之间无明显相关关系。结论慢性乙肝患者体内存在DC数量减少及成熟障碍,这种改变与肝内炎症反应程度不相关,但与乙肝病毒(HepatitisBvirus,HBV)的复制水平呈负相关,提示DC参与慢性乙肝患者体内HBV的清除。
Objective To investigate the changes of the number and phenotype of dendritic cells (DCs) derived from peripheral blood in patients with chronic hepatitis B, and to study their relationship with liver function and HBV replication. Methods The liver function and serum HBV DNA levels were determined in 37 patients with chronic hepatitis B and 21 healthy controls. Peripheral blood mononuclearcells (BMC) were extracted and cultured in vitro to induce their development into DC. The numbers of them were counted and the surface molecules The change. The relationship between DC quantity and phenotype and liver function and hepatitis B virus replication was analyzed. Results Compared with the normal control group, the number of dendritic cells in patients with chronic hepatitis B significantly decreased (P <0.05), and the expression of CD83 and CD86 on the membrane surface were significantly decreased (P <0.05). In patients with chronic hepatitis B, there is a negative correlation between the number of DCs, CD83 and CD86 on the surface of DCs and serum HBVDNA, but not between liver function. Conclusion There is a decrease in the number of DCs and maturation disorder in patients with chronic hepatitis B, which is not related to the degree of intrahepatic inflammatory reaction but negatively correlated with the replication level of Hepatitis B virus (HBV), suggesting that DC participates in the pathogenesis of HBV infection in patients with chronic hepatitis B Clear.