论文部分内容阅读
本文用地高辛标记cRNA探针原位杂交技术结合计算机回家分析，观察了在正常、伤害性刺激、单纯针刺和电针镇痛条件下大鼠脊髓背角内生长抑素mRNA的变化特点．结果如下：正常情况下脊髓背角内含有少量生长抑素mRNA阳性细胞，主要集中于Ⅱ、Ⅰ层．伤害性刺激，单纯针刺后背角生长抑素mRNA均明显增多，与其他三级相比，电针镇捕后背角生长抑索mRNA进一步增多，上述变化以Ⅱ层最为显著，且以同侧为甚。以上结果揭示生长抑素参与疼觉调制并且在针刺镇痛中起一定作用，其作用机制有待进一步研究．
In this paper, digoxigenin-labeled cRNA probe in situ hybridization combined with computer home analysis, observed under normal, nociceptive stimuli, simple acupuncture and electroacupuncture analgesia in rat spinal dorsal horn somatostatin mRNA changes . The results are as follows: Under normal circumstances spinal cord dorsal horn contains a small amount of somatostatin mRNA positive cells, mainly in Ⅱ, Ⅰ layer. Nociceptive stimuli and somatostatin mRNA of dorsal horn increased significantly after acupuncture. Compared with the other three groups, the mRNA expression of dorsal horn increased further after electroacupuncture, the most obvious change was in layer II, Side is staggering. The above results reveal that somatostatin is involved in modulation of pain and plays a role in acupuncture analgesia, and its mechanism needs further study.