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目的考察脑缺血模型大鼠与正常大鼠脑组织中(2-N-叔丁基硝酮)-3,5,6-三甲基吡嗪(TBN)的浓度差异。方法用线栓法,建立大脑中动脉栓塞脑缺血大鼠模型,按不同剂量或在不同再灌注时间后给药,用HPLC方法测定脑组织中TBN浓度。结果以不同剂量、在不同再灌注时间后给药,TBN在模型大鼠脑中的浓度均基本高于正常大鼠。模型鼠脑中TBN浓度随剂量增加而增大,两者呈良好的线性关系。于不同再灌注时间后给药,对脑中TBN浓度影响明显。结论在脑缺血再灌注后,TBN能更有效地透过大鼠血脑屏障,产生更高的脑药浓度。
Objective To investigate the difference of concentration of (2-N-tert-butyl nitrone) -3,5,6-trimethylpyrazine (TBN) in cerebral tissue between normal rats and rats with cerebral ischemia. Methods The rat model of middle cerebral artery occlusion with cerebral ischemia was established by thread suture method. The rats were administered with different dose or different reperfusion time, and the concentration of TBN in brain tissue was determined by HPLC. The results in different doses, administered at different reperfusion time, the concentration of TBN in model rat brain were basically higher than normal rats. The TBN concentration in brain of model rats increased with dose, showing a good linear relationship. After different reperfusion time after administration, the brain TBN concentration significantly affected. Conclusion TBN can penetrate the blood-brain barrier of rats more efficiently after cerebral ischemia and reperfusion, resulting in higher brain drug concentration.