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目的:定量研究睾酮分泌剧烈减少所致睾丸和附睾的组织学变化。方法:14只成年 SD 大鼠腹腔内注射二甲磺酸乙烷(EDS,75mg/kg),14只注射生理盐水作为对照。7天后处死各组中的一半动物,过5天后处死另一半。取睾丸和附睾组织块,甲基丙烯酸树脂包埋。用体视学的光学体视框技术估计睾丸内的细胞数,并用其它形态定量研究方法获取另外一些参数。结果:EDS 注射使睾丸内的间质细胞几乎完全消失,但对支持细胞总数没有影响。EDS 注射7天后,生精上皮内可见许多长形精子细胞滞留,附睾管内可见许多圆形精子细胞。EDS 注射12天后,精子细胞和精母细胞的排列明显变疏松,生精细胞之间出现明显的裂隙,裂隙近似放射状朝向生精小管腔;睾丸内的非 B 型精原细胞总数和精母细胞总数与对照组相似,但 B 型精原细胞总数增加59%,而早期(圆形)、中期和晚期(长形)精子细胞总数分别减少37%、72%和52%。结论:EDS 所致精子发生损害主要是(1)精子释放障碍,(2)精子细胞、精母细胞分离并伴有精子形成和成熟分裂障碍。
OBJECTIVE: To quantitatively study the histological changes of testis and epididymis caused by the dramatic decrease of testosterone secretion. Methods: Fourteen adult Sprague-Dawley rats were injected intraperitoneally with EDS (75 mg / kg) and 14 normal saline as controls. Half of the animals in each group were sacrificed 7 days later and the other half sacrificed after 5 days. Take testicular and epididymis blocks, methacrylic resin embedded. The number of cells in the testes is estimated using stereospectrophotometry and other morphological studies are used to obtain additional parameters. RESULTS: EDS injection almost completely eliminated interstitial cells within the testes, but had no effect on the total number of supporting cells. Seven days after EDS injection, many elongated spermatids were seen in the seminiferous epithelium. Many round sperm cells were found in the epididymis. After 12 days of EDS injection, the arrangement of spermatids and spermatocytes significantly loosens, and obvious slippage occurs between spermatogenic cells. The fissures are radial toward the seminiferous tubules. The total number of non-B spermatogonia and sperm The total number of cells was similar to that of the control group, but the total number of type B spermatogonia increased by 59% while the number of early, middle, and late (long) sperm cells decreased by 37%, 72% and 52%, respectively. Conclusion: The main causes of spermatogenesis caused by EDS are: (1) sperm release disorder; (2) spermatids and spermatocytes are separated with spermatogenesis and mature schizoid disruption.