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目的探讨三氯乙烯(TCE)4周静式吸入下对雄性大鼠所致DNA损伤。方法将TCE分为4个浓度组,对雄性大鼠静式吸入染毒,用~(32)后标法检测肝脏组织和外周血白细胞(WBC)DNA损伤。结果与对照组比较,剂量组放射自显影指纹图多2～3个斑点；1～4周各剂量组肝脏组织和外周血WBC TCE-DNA加合物含量增加,与对照组比较,差异均有统计学意义(P＜0．05)；各浓度组TCE- DNA加合物含量均随TCE染毒剂量量的增加而增加,呈一定的线性趋势和剂量-效应关系；经4周染毒后再饲养1周的大鼠肝脏组织和外周血WBC TCE-DNA加合物各浓度组间差异无统计学意义(P＞0．05)；同一剂量组1～4周肝脏组织TCE-DNA加合物含量均先升高后降低再升高,而外周血WBC TCE-DNA加合物含量同一剂量组各时间点差异无统计学意义(P＞0．05)；各剂量组肝脏组织和外周血WBC TCE-DNA加合物相关性良好。结论4周静式吸入TCE对受试动物有一定的DNA损伤。
Objective To investigate the DNA damage induced by trichlorethylene (TCE) in rats under static inhalation for 4 weeks. Methods TCEs were divided into four groups and were exposed to static inhalation. The DNA damage of liver and peripheral blood leukocytes (WBC) was detected by ~ (32) post-labeling method. Results Compared with the control group, the fingerprints of autoradiograph in the dose group were more than 2 to 3 spots. The contents of WBC TCE-DNA adduct in the liver tissue and peripheral blood in each dose group increased from 1 to 4 weeks. Compared with the control group, the difference was (P <0.05). The content of TCE-DNA adduct in each concentration group increased with the increase of TCE dose, showing a linear trend and dose-effect relationship. After 4 weeks of exposure There was no significant difference in the concentration of WBC TCE-DNA adducts between the re-fed 1 week rat liver tissue and the peripheral blood (P> 0.05). TCE-DNA addition of liver tissue from 1 to 4 weeks in the same dose group The content of WBC TCE-DNA adduct in the same dose group had no significant difference at each time point (P> 0.05). The liver tissue and peripheral blood of each dose group WBC TCE-DNA adduct was well correlated. Conclusions Four weeks of static inhalation of TCE have some DNA damage to the test animals.