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目的:检测血管内皮生长因子C(vascular endothelial growth factor C,VEGF-C)mRNA和蛋白在人乳腺癌细胞株MCF-7及其耐药株MCF-7/Adr中的定位、定性表达.方法:根据VEGF-C基因序列,设计合成地高辛标记的特异性寡核苷酸探针,运用原位杂交方法检测培养的细胞株MCF-7和MCF-7/Adr中VEGF-C mRNA的表达;并运用免疫组织化学方法检测了两种细胞中VEGF-C蛋白的表达.结果:原位杂交检测到MCF-7和MCF-7/Adr细胞的胞浆中有阳性蓝色颗粒,免疫组化检测发现两种细胞的胞浆中均有阳性棕黄色颗粒,而阴性对照细胞的胞浆中则均无阳性颗粒.结论:人乳腺癌细胞株MCF-7及其耐药株MCF-7/Adr细胞能够转录VEGF-C mRNA并在其细胞浆中翻译合成相应的蛋白.“,”Objective:The study was designed to examine the expressions of vascular endothelial growth factor C(VEGF-C) mRNA and protein in human breast cancer cell line MCF-7 and drug resistant cell line MCF 7/Adr.Methods:According to the VEGF-C gene sequence,we designed and constructed a specific oligonucleotide probe labeled with digoxin.Then in situ hybridization was employed to detect respectively the expression of VEGF-C mRNA in the cultured human breast cancer cell lines MCF-7 and MCF-7/Adr.In addition immunohistochemistry was employed to examine the expression of VEGF-C protein in both cell lines.Results:Blue positive stainning granules for VEGF-C mRNA were observed in the cytoplasm of these cells by in situ hybridization where brown positive staining granules for VEGF-C protein were also observed by immunohistochemistry.While there were no positive staining granules in neither control cells.Conclusion:Human breast cancer cell lines MCF-7 and MCF-7/Adr can transcribe VEGF-C mRNA and translate corresponding protein in their cytoplasm.