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目的了解徐州市副溶血性弧菌(VP)分布及病原学特征。方法对样品进行常规检测,血清学分型,药敏试验;PCR技术检测VP的TDH、TRH毒力基因。结果徐州市水产品VP检出率为57.50%(23/40),其中2株trh基因(+);23株VP中3株(7.50%,3/40)未定血清型,其余20株分布在7个血清型,有O1、2、3、4、5、10、11型;其中O1、O4、O5构成比为65.22%(15/23),是主要流行血清型。食源性腹泻患者粪便中VP检出率为8.00%(4/50),其中2株为O3:K6血清型且tdh基因(+)。水产品中VP的trh基因阳性率为8.70%(2/23),未检出tdh基因;食源性腹泻标本检出菌tdh基因阳性率为50.00%,trh基因未检出。水产品中检出的VP主要对替卡西林、阿莫西林、妥布霉素、头孢呋辛、头孢西丁耐药;腹泻患者粪便中检出的VP主要对阿莫西林和替卡西林耐药。结论徐州市水产品VP携带率高,食源性腹泻患者粪便中检出VP,且携带毒力基因;VP耐药情况较严重;不同病原学特征的VP引起的患者临床症状无明显差别。VP的防治工作中应进一步加强监测其血清型、耐药性以及携带毒力基因的情况;对腹泻患者的临床症状和VP的病原学特征之间的关系需进一步研究。
Objective To understand the distribution and etiological characteristics of Vibrio parahaemolyticus (VP) in Xuzhou City. Methods The samples were routinely tested, serologically typed and susceptible. PCR was used to detect VPH TDH and TRH virulence genes. Results The detection rate of VP in aquatic products in Xuzhou was 57.50% (23/40), including 2 trh genes (+), 3 strains (3.50%, 3/40) in 23 strains of undetermined serotypes and the remaining 20 strains in 7 serotypes, there O1,2,3,4,5,10,11 type; O1, O4, O5 constitute 65.22% (15/23), is the major epidemic serotypes. The detection rate of VP in feces of patients with food-borne diarrhea was 8.00% (4/50), of which 2 were O3: K6 serotypes and the tdh gene (+). The positive rate of trh gene in aquatic products was 8.70% (2/23), no tdh gene was detected. The positive rate of tdh gene in food-borne diarrhea samples was 50.00%, while trh gene was not detected. VP detected in aquatic products was predominantly resistant to ticarcillin, amoxicillin, tobramycin, cefuroxime, and cefoxitin; VPs detected in feces of patients with diarrhea were predominantly resistant to amoxicillin and ticarcillin medicine. Conclusion The VP carrying rate of aquatic products in Xuzhou City is high. VP is detected in feces of food-borne diarrhea patients and carries virulence genes. The VP drug resistance is more serious. There is no significant difference in the clinical symptoms caused by VP with different etiological characteristics. The prevention and treatment of VP should be further strengthened to monitor its serotype, drug resistance and virulence genes; the relationship between the clinical symptoms of VP and the etiological characteristics of VP needs to be further studied.