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探讨疣粒野生稻应答黄单胞杆菌水稻致病变种(Xoo)的基因芯片制作,通过芯片杂交筛选抗病相关基因。芯片含有2436个片段,来自于应答Xoo的疣粒野生稻差减文库和cDNA文库,通过芯片杂交及微阵列分析基因表达,选其中800个样品点测序比对。其中,35个无同源序列,大部分有同源序列的功能未知,已知功能的序列中明显上调表达的基因有:富含脯氨酸蛋白、泛素连接酶、伸展蛋白、谷胱甘肽S-转移酶II、脂类转移酶等,明显下调表达的基因有:细胞色素P450单加氧酶、醛缩酶、金属硫蛋白、硫氧还蛋白、热激蛋白等,表达无明显变化的基因有:抗坏血酸过氧化物酶、转铜伴侣、脂酶、花丝温敏H2A蛋白等。高通量基因芯片的利用及微阵列分析是筛选抗病相关基因、获取大量抗病相关信息的有效手段。
To investigate the gene chip production of Xoo by Xanthomonas oryzae, and to screen the disease-resistance-related genes by chip hybridization. The chip contains 2436 fragments from watered wild rice subtracted cDNA library and cDNA library in response to Xoo. The gene expression was analyzed by chip hybridization and microarray, and 800 of them were sequenced and aligned. Among them, 35 non-homologous sequences, most of which have homologous sequences, have unknown function. Genes with significantly up-regulated expression in known functional sequences are: proline rich protein, ubiquitin ligase, extensin, glutathione Peptide S-transferase II, lipid transferase and other genes were significantly down-regulated expression: cytochrome P450 monooxygenase, aldolase, metallothionein, thioredoxin, heat shock protein, no significant change in expression The genes are: ascorbate peroxidase, copper partner, lipase, filament thermo-sensitive H2A protein. The use of high-throughput microarray and microarray analysis are effective tools for screening disease-related genes and obtaining a large amount of disease-related information.