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目的:探讨加味茵陈四逆汤含药血清对转化生长因子-β1(TGF-β1)干预的大鼠肝星状细胞TGF-β1/Smad通道相关蛋白及胶原蛋白3(Collagen3)生成量的影响。方法:以大鼠肝星状细胞HSC-T6为研究对象,将细胞分为空白组,模型组,阳性药物组(甲强龙,10%含药血清),加味茵陈四逆汤高、中、低剂量组(10%含药血清),共6组。除空白组外,其余均给予TGF-β1(10 g·L-1)并采用相应10%的含药血清培养,并采用蛋白质免疫印迹(Western blot)的方法检测各研究组含药血清对大鼠肝星状细胞HSC-T6 Smad3,Smad7,Collagen3蛋白表达的影响。结果:与空白组比较,模型组HSC-T6细胞Smad3,Smad7,Collagen3蛋白表达升高,Collagen3蛋白表达升高较为明显(P<0.05);与模型组比较,加味茵陈四逆汤低剂量明显降低Smad3蛋白表达(P<0.05),加味茵陈四逆汤中剂量明显降低Collagen3蛋白表达(P<0.05)。结论:加味茵陈四逆汤含药血清使Smad3,Collagen3蛋白表达下调,Smad7未见明显变化,提示本药在离体条件下可以延缓肝纤维化的进程。
Objective: To investigate the effects of modified Yinchen Sini Decoction-containing serum on TGF-β1 / Smad channel-related protein and Collagen 3 production in rat hepatic stellate cells treated with TGF-β1 . Methods: Rat HSC-T6 cells were divided into blank group, model group, positive drug group (methylprednisolone, 10% drug-containing serum), Modified Yinchen Sini Decoction high, medium , Low dose group (10% drug serum), a total of 6 groups. In addition to the blank group, the rest were given TGF-β1 (10 g · L-1) and the corresponding serum containing 10% culture, and Western blot was used to detect the study group serum containing large Effects of HSC-T6 Smad3, Smad7 and Collagen3 Protein Expression in Rat Hepatic Stellate Cells. Results: Compared with the blank group, the expression of Smad3, Smad7 and Collagen3 in HSC-T6 cells increased and the expression of Collagen3 increased significantly in model group (P <0.05). Compared with the model group, the low dose of Jiaweiminqinqin Decoction Decreased the expression of Smad3 protein (P <0.05), and the moderate dose of Yinchen Sini Decoction significantly reduced the expression of Collagen3 protein (P <0.05). Conclusion: Modified Herba Sini Decoction containing serum Smad3, Collagen3 protein expression decreased, Smad7 no significant change, suggesting that the drug in vitro can delay the process of liver fibrosis.