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用液闪计数测定经组织培养的6株人肿瘤(MGC 80-3、SGC-7901、BEL-7402、Os-732、Detroit-6和 HeLa)细胞及1株正常成纤维细胞对~3H-HPD 的摄取和存留。MGC 80-3和成纤维细胞的摄取量,在测定的24h 内,随温育时间延长而增加;温育30 min 换液,再培养30 min,血卟啉衍生物含量就明显减少,MGC 80-3和成纤维细胞分别只含原摄入量的32.01%和40.75%;但在以后的24h 内,维持在类似的水平上。7株细胞温育30min 摄入~3H-HPD 量,按多少顺序依次为成纤维细胞、HeLa、BEL-7402、Detroit-6、SGC-7901、MGC 80-3 及 Os-732;但是,培养24h 后,由于各株细胞排出量不同,~3H-HPD 存留率大小顺序为 Os-732、Detroit-6、BEL-7402、HeLa、SGC-7901,MGC 80-3 及成纤维细胞。环境因素对细胞摄取~3H-HPD 起重要作用,提高 pH 值、加入血清或降低温度都使摄入减少。本文结果提示,恶性组织比正常组织血卟啉衍生物含量高,不是因为恶性细胞摄入多,而是由于存留时间长,与体内实验结果一致,可供 HPD 光敏治疗恶性肿瘤作参考。
The expression of ~ 3H-HPD in 6 human tumors (MGC 80-3, SGC-7901, BEL-7402, Os-732, Detroit-6 and HeLa) and 1 normal fibroblast Uptake and retention. The uptake of MGC 80-3 and fibroblasts increased with the incubation time within 24 hours of determination. After incubation for 30 minutes, the amount of hematoporphyrin derivative was significantly decreased after 30 minutes of incubation, and the amount of MGC 80-3 -3 and fibroblasts contained only 32.01% and 40.75% of the original intakes, respectively; however, they remained at similar levels for the next 24 hours. The number of ~ 3H-HPD uptake in 7 cells after 30min incubation were in order of fibroblasts, HeLa, BEL-7402, Detroit-6, SGC-7901, MGC 80-3 and Os-732; however, Afterwards, the survival rate of ~ 3H-HPD was Os-732, Detroit-6, BEL-7402, HeLa, SGC-7901, MGC 80-3 and fibroblasts, Environmental factors play an important role in the cellular uptake of ~ 3H-HPD, increasing the pH, decreasing the intake by adding serum or lowering the temperature. Our results suggest that malignant tissue hematoporphyrin derivatives higher than normal tissue content, not because of malignant cells uptake, but because of long retention time, consistent with in vivo experimental results for HPD photosensitive treatment of malignant tumors for reference.