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目的:建立不同产地狭叶柴胡地上部分黄酮类成分的高效液相特征色谱。方法:采用Kromasil C18(4.6mm×250mm,5μm)色谱柱,流动相为甲醇-0.5%醋酸水溶液,梯度洗脱,流速0.4mL·min-1,检测波长365nm,柱温40℃;并利用HPLC-ESI-TOFMS对主要共有色谱峰进行鉴定,采用电喷雾正离子源模式,N2流速7L·min-1,载气温度320℃,毛细管电压3kV,轰击电压100V,扫描质子范围m/z100～1000。结果:不同产地狭叶柴胡地上部分有14个共有色谱峰,各共有色谱峰之间的分离度良好;利用质谱信息与文献结合对共有色谱峰中的10个进行了结构确认。结论:利用建立的特征色谱可以对狭叶柴胡地上部分药材进行质量评价,为狭叶柴胡地上部分资源的综合利用提供了科学依据。
Objective: To establish a high performance liquid chromatography (HPLC) characteristic chromatogram of flavonoids from aerial parts of Bupleurum chinense from different habitats. METHODS: A Kromasil C18 (4.6 mm×250 mm, 5 μm) column was used. The mobile phase consisted of methanol-0.5% acetic acid aqueous solution, gradient elution, flow rate of 0.4 mL·min-1, detection wavelength at 365 nm, column temperature at 40°C, and HPLC. The ESI-TOFMS was used to identify the main common peaks. The electrospray positive ion source mode was adopted. The N2 flow rate was 7L·min-1, the carrier gas temperature was 320°C, the capillary voltage was 3kV, the bombardment voltage was 100V, and the scanning proton range was m/z 100-1000. . RESULTS: There were 14 common chromatographic peaks in the aerial parts of Bupleurum chinense from different habitats. The separation between the common chromatographic peaks was good. The identification of 10 of the common peaks was confirmed by the combination of mass spectrometry information and literature. Conclusion: The quality index of the aerial parts of Radix Bupleurum and Radix Bupleurum can be evaluated by the established characteristic chromatogram, which provides a scientific basis for the comprehensive utilization of the aerial parts of Bupleurum chinense.