Recently,multifunctional polymers for protein conjugation have been facilely synthesized through the multicomponent reactions(MCRs).In this mini-review,current progress in the generation of multifunctional polymers with protein-reactive groups via MCRs is summarized.These multifunc-tional polymers react with model therapeutic proteins,forming multifunctional polymer-protein conjugates,which are prototypes of sophisticated theranostic agents and antibacterial vaccine candidates.In comparison with the traditional multi-step synthesis,the preparation of multifunctional polymers for protein conjugation through MCRs is straightforward and convenient.Due to these properties,MCRs have the potential to become a new general strategy to achieve polymer-protein conjugates for biological and medical applications.