论文部分内容阅读
本文报道云南山茶花(Camellia reticulataL.)的茎尖培养诱导形成多芽体和生根的条件及影响因素。MS培养基中含有较高浓度(3—5 mgL~(-1))BA和适量(1—1.5 mgL~(-1))IAA或NAA诱导形成多芽体。外植体的母株年龄明显影响多芽体的形成,幼龄种苗外植体的多芽诱导率高于成年树。添加CM(椰乳)、ZT、尿囊素对成年芽条返幼态有明显的促进作用。采用“浸没-纸桥生根法”诱根,以高浓度生长调节剂(0.5gL~(-1)IBA或ABT生根粉)溶液浸芽条基部短时间(20—30 min),生根效果最佳。母树的年龄对芽条的生根也有显著影响,种苗来源的芽条的生根率和根的数目都比来源于成年树的高。生根培养基中蔗糖浓度为15—20 gL~(-1),生根率高。MW和1/2 ER培养基比MS培养基的生根效果好。试管植物移栽入土后生长正常并已开花。
This paper reports the conditions and influential factors of shoot formation induced by shoot emergence in Camellia reticulataL. MS medium containing higher concentrations (3-5 mgL -1) of BA and appropriate amount of IAA or NAA (1-1.5 mgL -1) induces the formation of multi - buds. The mother plant age of explants obviously affected the formation of multi-buds. The induction rate of multiple buds of young seedling explants was higher than that of adult tree. Adding CM (coconut milk), ZT, allantoin on juvenile juvenile buds had a significant role in promoting. The roots were rooted with “Immersion - Paper Bridge Rooting” method and rooted at the base of buds with high concentration of growth regulator (0.5gL -1 IBA or ABT rooting powder) for a short time (20-30 min) . The age of the mother tree also had a significant effect on the rooting of the shoots, and the rooting rate and root number of the shoots originating from the seedling were higher than those derived from the adult tree. The rooting medium had a sucrose concentration of 15-20 gL ~ (-1) and high rooting rate. MW and 1/2 ER medium had better rooting effect than MS medium. In vitro test tube plants grow normally after planting and have flowered.