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目的 探讨基因重组人类肿瘤坏死因子 α(rh TNF- α)和 bcl- 2反义寡核苷酸 (ASPO)对 HL- 6 0细胞增殖与凋亡的作用 ,进一步认识肿瘤坏死因子 α(TNF- α)和 bcl- 2两者调控肿瘤细胞凋亡的作用及相互联系。 方法 单独和联合应用 rh TNF- α和 bcl- 2 ASPO处理 HL- 6 0细胞 ,通过 MTT法检测 HL- 6 0细胞生长和存活特性变化 ;原位细胞凋亡检测和流式细胞技术检测细胞凋亡 ;应用 RT- PCR、免疫组织化学检测癌基因 bcl- 2表达的改变。 结果 rh TNF- α和 bcl- 2 ASPO的联合应用明显增强对 HL- 6 0细胞的增殖抑制 ;细胞凋亡率明显升高 ;癌基因bcl- 2在 m RNA和蛋白表达明显下降。 结论 TNF和 bcl- 2 ASPO在诱导 HL- 6 0细胞凋亡中可能存在协同作用 ,为血液肿瘤的联合治疗提供了新的途径
Objective To investigate the effects of recombinant human tumor necrosis factor α (rh TNF-α) and bcl-2 antisense oligonucleotide (ASPO) on the proliferation and apoptosis of HL-60 cells, and to further understand the role of tumor necrosis factor- α) and bcl-2 both regulate tumor cell apoptosis and their relationship. Methods HL-60 cells were treated with rh TNF-α and bcl-2 ASPO alone or in combination. The growth and survival characteristics of HL-60 cells were detected by MTT assay. In situ cell apoptosis assay and flow cytometry The changes of oncogene bcl-2 expression were detected by RT-PCR and immunohistochemistry. Results The combination of rh TNF-α and bcl-2 ASPO significantly enhanced the proliferation of HL-60 cells. The apoptosis rate was significantly increased. The expression of oncogene bcl-2 was significantly decreased in m RNA and protein. Conclusion TNF and bcl-2 ASPO may play synergistic roles in inducing HL-60 cell apoptosis and provide a new approach for the combination therapy of hematological tumors