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目的 探讨纳洛酮对急性心肌缺血面积的影响。方法 结扎冠状动脉左室支中点复制心肌缺血模型。采用纳洛酮干预 ,缺血 3个小时后静脉注射美兰使非缺血区兰染 ,处死动物分离左心室 (LV)并切片 ,用TTC染色 ,区分缺血心肌(IS)、危险心肌 (AAR)及正常心肌。在解剖显微镜下分离IS、AAR ,分别称重 ,观察IS、AAR、IS/AAR %及IS/LV %以评价缺血范围。结果 纳洛酮实验组IS、AAR、IS/LV %、IS/AAR %明显减少与对照组相比差异显著。结论 纳洛酮能明显缩小急性心肌缺血的缺血面积。其机理可能与改善心肌微循环、促进组织氧合、降低组织乳酸浓度、减少缺血区水肿、防止膜脂质过氧化、对心肌缺血及缺血后的继发性损伤的抑制作用等有关
Objective To investigate the effect of naloxone on acute myocardial ischemia area. Methods Ligation of coronary artery left ventricular midpoint of myocardial ischemia model. Naloxone was used to intervene. After 3 hours of ischemia, melanin was intravenously injected into the non-ischemic area and left ventricular (LV) was sacrificed and sliced. TTC staining was performed to distinguish ischemic myocardium (IS) AAR) and normal myocardium. IS and AAR were isolated under a dissecting microscope and weighed respectively. IS, AAR, IS / AAR% and IS / LV% were observed to evaluate the extent of ischemia. Results Naloxone experimental group IS, AAR, IS / LV%, IS / AAR% significantly reduced compared with the control group significant difference. Conclusion Naloxone can significantly reduce the ischemic area of acute myocardial ischemia. The mechanism may be related to improving myocardial microcirculation, promoting tissue oxygenation, reducing tissue lactic acid concentration, reducing ischemic edema, preventing membrane lipid peroxidation, and inhibiting myocardial ischemia and secondary damage after ischemia and so on