小鼠肝癌腹水瘤细胞HCa/A2 -P用含与不含异搏定的培养基体外培养 96h ,利用钙调素对环核苷酸磷酸二酯酶的激活作用测定胞液钙调素含量 ,用药组钙调素平均浓度为 19 1μg/mg蛋白 (n1=6 ) ,对照组为 2 5 6 μg/mg蛋白 (n2 =6 ) ,用药组胞液钙调素含量明显降低 (P <0 0 0 5) ,提示异搏定可能通过影响Ca2 + /钙调素信号途径来影响此肿瘤细胞的生理过程。 Mouse hepatocellular carcinoma ascites tumor cells HCa / A2-P with and without the verapamil culture medium in vitro 96h, the use of calmodulin cyclic nucleotide phosphodiesterase activation cytosolic calmodulin content, The average concentration of calmodulin in the treatment group was 19 1 μg / mg protein (n1 = 6), while the control group was 2.56 μg / mg protein (n2 = 6). The content of cytochalasin in the treatment group was significantly decreased (P <0 0 0 5), suggesting that verapamil may affect Ca2 + / calmodulin signaling pathway to affect the physiological process of this tumor cells.