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目的:评价荧光原位杂交技术(FISH)在膀胱肿瘤诊断中的应用价值。方法:20例正常人通过FISH技术检测9号染色体P16位点、3号染色体、7号染色体及17号染色体的变异情况,建立阈值。20例血尿患者留取晨尿,分别进行尿脱落细胞学检查和FISH检查。以至少两种探针检测结果超过阈值或一种探针检测结果至少存在两种异常为诊断阳性。结果:20例血尿患者除1例为泌尿系结石、1例为BPH外,病理检查证实的18例膀胱肿瘤患者中,FISH检出16例,尿脱落细胞学检出4例。结论:FISH无创快速,检出率高,在膀胱肿痛早期诊断中优于尿脱落细胞学检查方法。
Objective: To evaluate the value of fluorescence in situ hybridization (FISH) in the diagnosis of bladder cancer. Methods: Twenty normal controls were used to detect the variation of P16, chromosome 3, chromosome 7 and chromosome 17 on chromosome 9 by FISH. Twenty patients with hematuria were enrolled in morning urine, and urine cytology and FISH were performed respectively. There are at least two abnormalities that are diagnostic positive with at least two probe test results exceeding a threshold or one probe test result. Results: Of the 20 patients with hematuria, one was urinary calculi and one was BPH. Of the 18 patients with bladder cancer confirmed by pathology, 16 were detected by FISH and 4 were detected by exfoliative cytology. Conclusion: FISH is noninvasive and rapid with high detection rate. It is superior to urinary cytology in the early diagnosis of bladder swelling.