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目的研究螺内酯、氯沙坦对大鼠心肌梗死后血流动力学和心肌胶原重建的影响。方法于2005年10月至2006年3月在河北省医科院药理实验室,结扎大鼠冠状动脉左前降支制作心肌梗死模型,随机分为假手术组、安慰剂组、螺内酯组、氯沙坦组、螺内酯+氯沙坦组。第2、6周观察平均动脉压(MBP)、左室舒张末压(LVEDP)、左室相对重量、非梗死区心肌胶原含量及Ⅰ/Ⅲ型胶原蛋白比值。结果安慰剂组较假手术组LVEDP升高,心肌胶原含量及Ⅰ/Ⅲ型胶原蛋白比值增加。第2周,氯沙坦组和螺内酯+氯沙坦组较安慰剂组MBP、LV-EDP、心肌胶原含量及Ⅰ/Ⅲ型胶原蛋白比值下降。第6周,螺内酯组、氯沙坦组及螺内酯+氯沙坦组与安慰剂组相比较LVEDP、心肌胶原含量及Ⅰ/Ⅲ型胶原蛋白比值均下降,螺内酯+氯沙坦组较螺内酯组、氯沙坦组心肌胶原含量降低更显著。结论螺内酯、氯沙坦均可改善心肌梗死后血流动力学,抑制胶原增生。两药联合应用可充分抑制胶原重建作用。
Objective To investigate the effects of spironolactone and losartan on hemodynamics and myocardial collagen after myocardial infarction in rats. Methods From October 2005 to March 2006, a myocardial infarction model was established in the left anterior descending coronary artery of rats in the Hebei Medical Laboratory of Chinese Academy of Sciences from October 2005 to March 2006, and randomly divided into sham operation group, placebo group, spironolactone group, losartan Group, spironolactone + losartan group. The mean arterial pressure (MBP), left ventricular end-diastolic pressure (LVEDP), left ventricular relative weight, myocardial collagen content in non-infarcted area and ratio of type Ⅰ / Ⅲ collagen were observed at the 2nd and 6th week. Results Compared with sham-operation group, LVEDP increased, myocardial collagen content and type Ⅰ / Ⅲ collagen ratio increased in placebo group. In the second week, the ratio of MBP, LV-EDP, myocardial collagen and type Ⅰ / Ⅲ collagen decreased in losartan group, spironolactone + losartan group and placebo group. Compared with placebo group, LVEDP, myocardial collagen content and type Ⅰ / Ⅲ collagen ratio decreased in spironolactone group, losartan group, spironolactone + losartan group and spironolactone group, Losartan group myocardial collagen content decreased more significantly. Conclusion Spironolactone and Losartan can both improve hemodynamics and inhibit collagen hyperplasia after myocardial infarction. The combination of two drugs can fully inhibit collagen remodeling.