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目的探讨调节性T细胞(regulatory T cell,Treg)水平变化与肾移植急性排斥反应之间的关系。方法选取54例肾移植受者(其中男26例,女28例),根据术后12周内是否发生急性排斥反应分为急性排斥组和稳定组,于术前1-2d、术后1周、4周、8周、12周分别取外周静脉血样本,应用流式细胞仪检测外周血中Treg细胞水平。结果术前第1-2d,急性排斥组和稳定组的Treg细胞水平比较有明显差异(P<0.05)。术后4周内两组Treg细胞水平均急剧下降。术后第4-8周,稳定组受者的Treg细胞水平开始回升,而急性排斥组继续下降。术后第8-12周,稳定组的Treg细胞水平明显回升,但排斥组则仍维持在较低水平,且此阶段两组Treg细胞水平的差别具有统计学意义(P<0.05)。结论外周血中的Treg细胞水平的检测可协助预测肾移植急性排斥反应发生的高危群体,有助于术后急性排斥反应的早期诊断。
Objective To investigate the relationship between the level of regulatory T cell (Treg) and the acute rejection of renal allograft. Methods Fifty-four renal transplant recipients (26 males and 28 females) were divided into acute rejection group and stable group according to whether acute rejection occurred within 12 weeks after operation, , 4 weeks, 8 weeks, 12 weeks were taken peripheral venous blood samples were detected by flow cytometry Treg cells in peripheral blood levels. Results The levels of Treg cells in acute rejection group and stable group were significantly different (P <0.05) 1-2 days before operation. After 4 weeks, Treg cells in both groups dropped sharply. After 4-8 weeks, Treg cells in stable recipients began to rise, while those in acute rejection continued to decrease. At 8-12 weeks after operation, the level of Treg cells in the stable group increased significantly, but the levels in the rejection group remained at a low level. The difference of Treg cells between the two groups was statistically significant (P <0.05). Conclusion The detection of Treg cells in peripheral blood can be used to predict the high risk group of acute rejection after renal transplantation, which is helpful for the early diagnosis of postoperative acute rejection.