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目的研究漯河周边地区非小细胞肺癌(non small cell lung cancer,NSCLC)病变组织中HPV16E6、E7蛋白表达情况及与临床病理参数之间的关系,探讨HPV16E6和E7蛋白在NSCLC发病机制中的作用。方法 2010年3月—2011年6月采用免疫组织化学技术分别检测80例NSCLC标本和55例癌周对照组织中HPV16E6和E7蛋白的表达,并进行对比,计数资料采用χ2检验或确切概率法,P<0.05为差异有统计学意义。结果 80例NSCLC组织中,HPV16 E6、E7、E6/E7蛋白的表达率分别为31.3%、33.8%和28.8%,55例癌旁组织中,HPV16E6、E7、E6/E7蛋白表达阳性率分别为14.5%、9.2%、7.3%。NSCLC组织中E6、E7和E6/E7蛋白共表达阳性率均高于对照组,差异有统计学意义(P<0.05),与患者年龄、性别以及肿瘤组织学类型无关,但与肿瘤分化以及淋巴结转移有关。结论 HPV16E6和E7蛋白在NSCLC发生发展中具有重要作用。
Objective To investigate the relationship between the expression of HPV16E6 and E7 protein and the clinicopathological parameters in non small cell lung cancer (NSCLC) in the surrounding areas of Luohe and to explore the role of HPV16 E6 and E7 proteins in the pathogenesis of NSCLC. Methods From March 2010 to June 2011, immunohistochemistry was used to detect the expression of HPV16E6 and E7 proteins in 80 specimens of NSCLC and 55 specimens of peri-cancerous lesions respectively. The count data were analyzed by χ2 test or exact probability method. P <0.05 for the difference was statistically significant. Results The positive rates of HPV16 E6, E7, E6 / E7 protein expression in 80 NSCLC tissues were 31.3%, 33.8% and 28.8%, respectively. The positive rates of HPV16E6, E7 and E6 / E7 protein expression in 55 paracancerous tissues were 14.5%, 9.2%, 7.3%. The positive rates of E6, E7 and E6 / E7 protein expression in NSCLC tissues were significantly higher than those in control group (P <0.05), but not with the age, sex and tumor histological type, but were positively correlated with tumor differentiation and lymph node Transfer related. Conclusion HPV16 E6 and E7 proteins play an important role in the development of NSCLC.