利用心传导系统中的浦肯野氏纤维糖原含量较高的特点,而浦氏纤维仅位于薄层心内膜下,故用卢戈碘液涂布在心膜上,使碘液与糖原结合,产生棕褐色的颜色反应,这种染色法简便易行,能充分地显示出心内膜下浦氏纤维细网,对教学与科研都有一定实用意义。其操作方法如下:1.取材法:要用死后1～4小时以内的新生儿心脏或动物心脏(牛、狗、猪心), The use of cardiac conduction system of Purkinje fiber glycogen content is high, while Pu Fibers only in the thin layer of endocardium, it is coated with Lugo iodine solution in the pericardium, iodine and glycogen Combined to produce a tan color reaction, this staining method is simple and easy to fully display the sub-subendocardial fiber Fine fiber, the teaching and research have a certain practical significance. The method of operation is as follows: 1. Method: To use the newborn within 1 to 4 hours after death heart or animal heart (cow, dog, pig heart)