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目的建立多波长HPLC法同时测定养血清脑颗粒中没食子酸,原儿茶酸,绿原酸,咖啡酸,芍药苷,芍药内酯苷和阿魏酸的量。方法采用Aglient Zorbax SB-C18色谱柱(4.6 mm×250 mm,5μm),以0.08%磷酸水溶液-乙腈为流动相,梯度洗脱,体积流量0.95 mL/min,检测波长为280 nm(0～17 min),320 nm(17～25 min),230 nm(25～33 min),320 nm(33～43 min),柱温30℃。结果没食子酸,原儿茶酸,绿原酸,咖啡酸,芍药苷,芍药内酯苷,阿魏酸的线性范围分别为0.942～9.42μg/mL,0.408～4.08μg/mL,1.01～10.1μg/mL,0.332～3.32μg/mL,3.24～32.4μg/mL,7.34～73.4μg/mL,0.607～6.07μg/mL(r≥0.999 8);平均回收率(n=6)均在95%～105%范围内。结论所建立的HPLC法简便,灵敏,准确,可用于养血清脑颗粒质量控制。
OBJECTIVE To establish a multi-wavelength HPLC method for simultaneous determination of gallic acid, protocatechuic acid, chlorogenic acid, caffeic acid, paeoniflorin, paeoniflorin and ferulic acid in Yangxue Qingnao Granules. Methods Aglient Zorbax SB-C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase consisted of 0.08% phosphoric acid and acetonitrile with a gradient of 0.95 mL / min. The detection wavelength was 280 nm min), 320 nm (17-25 min), 230 nm (25-33 min) and 320 nm (33-43 min) respectively. The column temperature was 30 ℃. Results The linear ranges of gallic acid, protocatechuic acid, chlorogenic acid, caffeic acid, paeoniflorin, paeoniflorin and ferulic acid were 0.942-9.42μg / mL, 0.408-4.08μg / mL and 1.01-10.1μg / mL, 0.332 ~ 3.32μg / mL, 3.24 ~ 32.4μg / mL, 7.34 ~ 73.4μg / mL, 0.607 ~ 6.07μg / mL (r≥0.9998); average recovery (n = 6) 105% range. Conclusion The established HPLC method is simple, sensitive and accurate and can be used for the quality control of Yangxue Qingnao Granules.