逆转录病毒常被作为载体,用于目的蛋白的表达或目的基因的嵌合。尽管,实验室选用的都是非感染性病毒,但并不能排除这些病毒不会对人类造成伤害。逆转录病毒的监督和检测具有非常重要的意义。逆转录酶活性又是逆转录病毒存在活性的重要标志。因此,本文将有关逆转录病毒及逆转录酶检测的方法做一综述,望对研究者有参考意义。 Retroviruses are often used as vectors for the expression of a protein of interest or for the chimeric gene of interest. Although labs use non-infectious viruses, it does not rule out that these viruses do not harm humans. The surveillance and testing of retroviruses is of great importance. Reverse transcriptase activity is an important marker of retroviral activity. Therefore, this article will retrospectively review the methods of retrovirus and reverse transcriptase detection, which is of great value to researchers.