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目的研究丹参水溶性成分———丹参酚酸B镁对人主动脉内皮细胞生长增殖的影响和对自由基损伤内皮细胞的影响 ,以进一步了解丹参酚酸B镁的心血管药理作用机理。方法实验中使用第 3～ 6代的人主动脉内皮细胞 (humanaorticendothelialcell,HAEC) ,采用形态学和MTT法观察不同浓度药物对细胞增殖的影响 ;采用葡萄糖 -葡萄糖氧化酶造成的氧自由基损伤细胞模型观察药物的作用 ;透射电镜观察细胞超微结构的变化。结果丹参酚酸B镁 1 0mg/ml和 0 5mg/ml两个剂量对HACE有明显的细胞毒作用(P <0 0 1)。低于 0 2mg/ml的浓度对细胞增殖无不良影响 ;丹参酚酸B镁对自由基损伤细胞有明显的保护作用。电镜结果显示其可以保护线粒体等细胞超微结构。结论丹参酚酸B镁对HAEC细胞毒性较低 ;对自由基损伤细胞有明显的保护作用
Objective To study the effect of Salvia miltiorrhiza B magnesium on the proliferation and proliferation of human aortic endothelial cells and the effect of free radical-damaged endothelial cells on the cardiovascular pharmacological mechanism of salvianolic acid B magnesium. METHODS: Human aortic endothelial cells (HAEC) from the 3rd to 6th generation were used in the experiment. Morphological and MTT assays were used to observe the effects of different concentrations of drugs on cell proliferation; oxygen free radicals were damaged by glucose-glucose oxidase. The model was used to observe the effect of the drug; transmission electron microscopy was used to observe the changes of the ultrastructure of the cells. Results Salvia miltiorrhiza B Magnesium 10 mg/ml and 0 5 mg/ml had significant cytotoxic effects on HACE (P <0 01). The concentration below 02 mg/ml had no adverse effects on cell proliferation; Salvia miltiorrhiza B magnesium had obvious protective effects on free radical-damaged cells. Electron microscopy showed that it could protect the ultrastructure of mitochondria and other cells. Conclusion Salvia miltiorrhiza B magnesium has lower cytotoxicity on HAEC cells; it has obvious protective effect on free radical-damaged cells.