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目的 :探讨端粒酶活性表达、HP感染和胃癌发生的相关性。方法 :对 1 1 6例活检标本及 42例手术标本采用快速尿素酶法及组织 Giemsa染色进行 HP检测 ,同时留取标本采用端粒重复序列扩增 -银染法进行端粒酶活性的检测。结果 :HP阳性率在正常胃粘膜、慢性萎缩性胃炎、肠上皮化生及胃腺癌中逐渐升高 ,阳性率分别为 35 .7%、5 0 %、5 0 %、62 .5 %、68.6% ,胃癌组与正常胃粘膜组差异具有显著性 ,端酶活性在正常胃粘膜中阴性 ,在胃癌中为 88.6% ,明显高于慢性萎缩性胃炎 ( 30 .6% )、肠上皮化生 ( 37.5 % )及不典型增生 ( 37.5 % ) ,差异具有显著性 ,在胃癌中端粒酶的活性与有无淋巴结转移有关 ,且 HP阳性胃癌组端粒酶阳性率 ( 97.9% )明显高于 HP阴性组 ( 67.8% ) ,差异具有显著性。结论 :1端粒酶活性表达出现在慢性萎缩性胃炎、肠上皮化生及不典型增生癌前病变中及高表达于胃癌中 ,提示其与胃癌的发生发展有关。 2端粒酶的激活可能与 HP感染有关。
Objective: To investigate the correlation between telomerase activity, HP infection and gastric carcinogenesis. Methods: Totally 116 cases of biopsy samples and 42 cases of surgical specimens were examined by rapid urease assay and tissue Giemsa staining. Meanwhile, telomerase activity was detected by telomere repeated-sequence amplification-silver staining. Results: The positive rate of HP was gradually increased in normal gastric mucosa, chronic atrophic gastritis, intestinal metaplasia and gastric adenocarcinoma, the positive rates were 35.7%, 50%, 50%, 62.5% and 68.6 %. There was significant difference between gastric cancer group and normal gastric mucosa group. The telomerase activity was negative in normal gastric mucosa, which was 88.6% in gastric cancer, which was significantly higher than that in chronic atrophic gastritis (30.6%), intestinal metaplasia 37.5%), and atypical hyperplasia (37.5%). The difference of telomerase activity in gastric cancer was related to the presence or absence of lymph node metastasis, and the positive rate of telomerase in HP positive gastric cancer was significantly higher than that in HP Negative group (67.8%), the difference was significant. Conclusion: 1 telomerase activity is expressed in chronic atrophic gastritis, intestinal metaplasia and dysplasia precancerous lesions and high expression in gastric cancer, suggesting that the telomerase activity is related to the occurrence and development of gastric cancer. 2 telomerase activation may be related to HP infection.