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目的 观察雪旺细胞源神经营养因子在大鼠生长发育腰髓中的表达。方法 取不同年龄的胎鼠、幼鼠及成年鼠的腰髓或相关组织 ,行常规免疫组织化学研究。结果 雪旺细胞源神经营养因子在成年大鼠腰髓的整个白质和灰质后角Ⅲ、Ⅳ、Ⅴ层有较低表达。雪旺细胞源神经营养因子在胎龄 1 4、1 5d开始有表达 ,胚胎时期表达先增高后下降 ,其中胎龄 2 1d最高。出生后雪旺细胞源神经营养因子 (SDNF)在腰髓中表达明显下降 ,其中第 7、1 2d稍高。结论 SDNF在成年大鼠腰髓 (包括背根神经节 )有较低表达。SDNF在生长发育的腰髓中表达基本呈逐渐下降趋势。SDNF对大鼠脊髓的发育可能起促进作用
Objective To observe the expression of Schwann cell-derived neurotrophic factor (BDNF) in the rat lumbar spinal cord. Methods The lumbar spinal cord or related tissues of fetal rats, young rats and adult mice of different ages were taken for routine immunohistochemistry. Results Schwann cell-derived neurotrophic factor (BDNF) was found to be low expressed in the layers III, IV and Ⅴ of the whole white matter and gray matter posterior horn of adult rat lumbar spinal cord. Schwann cell derived neurotrophic factor expression in gestational age 1 4,1 5d, the expression of embryonic first increased and then decreased, of which the highest gestational age 21d. After birth, Schwann cell source neurotrophic factor (SDNF) was significantly decreased in the lumbar spinal cord, of which the 7th and 12th days were slightly higher. Conclusion SDNF is lower expressed in the lumbar spinal cord (including dorsal root ganglion) in adult rats. The expression of SDNF in the development of the lumbar spinal cord showed a gradual downward trend. SDNF may promote the development of spinal cord in rats